Liang Cheng, Director of Anatomic Pathology and Director of Molecular Pathology at Lifespan Academic Medical Center and Vice Chair for Translational Research at the Warren Alpert Medical School of Brown University, shared a post on LinkedIn about a recent article he and his colleagues co-authored, adding:
“Do you know how to FISH?
I am delighted to share our 20+ years of experience with FISH, just published in Human Pathology (PMID: 40782985) – Everything You Need to Know About FISH!
This comprehensive review covers the full spectrum of fluorescence in situ hybridization (FISH) applications from HER2 FISH interpretation and 1p/19q deletion cutoffs to emerging applications, future directions, and the role of AI.
On a personal note, this paper has been two decades in the making. I began writing it nearly 20 years ago, when my late colleague and friend, Dr. John Eble, asked me to establish a molecular lab and implement FISH testing at Indiana University. Seeing it finally published is deeply gratifying. It’s true that it can take a decade (or two!) to write a comprehensive review and I’m thrilled that it’s finally out. I hope you find it a valuable and practical resource.
This review provides practical guidelines for the clinical application of interphase FISH in diagnostic surgical pathology. It discusses key technical considerations – including tissue preparation, interpretation thresholds, and limitations – and highlights selected diagnostic applications relevant to daily surgical pathology practice.
The central dogma that abnormal chromosomal DNA determines a diseased cell’s phenotype continues to have powerful clinical utility for tumor diagnosis and treatment. FISH remains a versatile, cost-effective, and rapid tool for detecting chromosomal alterations.
Even as technology evolves, pathologists must remain mindful of FISH’s underlying chemistry, potential artifacts, and clinical context. It continues to be an indispensable and irreplaceable adjunct in surgical pathology for resolving diagnostic dilemmas and identifying therapeutic targets.”
Title: Genomic aberration detection by fluorescence in situ hybridization
Authors: Liang Cheng, Darrell D. Davidson, Shaobo Zhang
Read the Full Article on Human Pathology
More posts featuring Liang Cheng.
