Sudha Kumari, Assistant Professor at Indian Institute of Science (IISc), shared a post on LinkedIn:
“Thrilled to share our recent work in EMBOReports that uncovers actin waves in lymphocytes, which rescue antigen receptor loss during immune response. Super thankful to DBT/WT India Alliance, Anusandhan National Research Foundation (ANRF), Infosys, MOE-STARS.
After successful antigen recognition at the immunological synapse, some antigen receptor translocates to the cell center for downregulation, while a sizeable fraction is rescued for future engagement. The mechanism of sorting and rescue is not clear.
We used TIRF-SIM to observe the first two minutes of receptor action at the immunological synapse, and found that T cells generated outward actin waves, and used them to “kick” antigen receptors outwards, away from the downregulation zone
coupling of antigen receptor to outward waves required WASP protein, in the absence of which, although actin waves formed, most antigen receptor still ended up in the downregulation zone
While actin waves were seen in practically every primary lymphocyte type we tested, they were missing in the Jurkat T cell line. As a result, most antigen receptors ended up in the central downregulation zone here as well
Overall, we present a novel mechanism of actin-based directional sorting of surface receptors. How outward waves can co-exist with the famous centripetal flow of actin, and why primary cells have robust actin waves will be a focus of future work.
Many thanks to the facilities Indian Institute of Science (IISc) and the collaborators for making it possible. I also cannot say enough good things about the review process at EMBOReports: the reviewers were fair, critical, and their suggestions were very constructive. Many thanks!!”
Michael Dustin, Director of Research at Kennedy Institute of Rheumatology, shared this post, adding:
“Congratulations to Sudha Kumari and team for most detailed analysis of TCR dynamics at immunological synapse to date, leading to new insights!”
Title: Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse
Authors: Aheria Dey, Samuel Z Khiangte, Srishti Mandal, Huw Colin-York, Marco Fritzsche, Sumantra Sarkar, Sudha Kumari
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