Sendurai Mani: Reflection on EMT, Stemness, And Cellular Heterogenety
Sendurai Mani/vivo.brown.edu

Sendurai Mani: Reflection on EMT, Stemness, And Cellular Heterogenety

Sendurai Mani, Associate Director of Translational Oncology, Deanโ€™s Chair of Translational Oncology, and Professor of Medicine at Brown University, and Co-Founder and CSO of Iylon Precision Oncology, shared a post onย LinkedIn:

“๐—ฅ๐—ฒ๐—ณ๐—น๐—ฒ๐—ฐ๐˜๐—ถ๐—ผ๐—ป๐˜€ ๐—ผ๐—ป ๐—˜๐— ๐—ง, ๐—ฆ๐˜๐—ฒ๐—บ๐—ป๐—ฒ๐˜€๐˜€, ๐—ฎ๐—ป๐—ฑ ๐—–๐—ฒ๐—น๐—น๐˜‚๐—น๐—ฎ๐—ฟ ๐—›๐—ฒ๐˜๐—ฒ๐—ฟ๐—ผ๐—ด๐—ฒ๐—ป๐—ฒ๐—ถ๐˜๐˜†

Many years ago, I discovered that activation of the epithelial-to-mesenchymal transition (EMT) program can endow cancer cells with stem cellโ€“like properties. You can hear Bob Weinbergโ€™s reflections on how this idea emerged and evolved. This finding had a profound impact; this paper has now been ๐—ฐ๐—ถ๐˜๐—ฒ๐—ฑ ๐—ผ๐˜ƒ๐—ฒ๐—ฟ ๐Ÿญ๐Ÿฌ,๐Ÿฌ๐Ÿฌ๐Ÿฌ ๐˜๐—ถ๐—บ๐—ฒ๐˜€ (๐—š๐—ผ๐—ผ๐—ด๐—น๐—ฒ ๐—ฆ๐—ฐ๐—ต๐—ผ๐—น๐—ฎ๐—ฟ).

In many carcinomas, we see cells with mesenchymal morphology that are not highly aggressive; similarly, when EMT is experimentally induced, not every cell gains stem-like traits. This observation raises a deeper biological question: Is there heterogeneity within the EMT-induced population?

In other words, ‘๐˜ฟ๐™ค๐™š๐™จ ๐™€๐™ˆ๐™ ๐™ฅ๐™ง๐™ค๐™™๐™ช๐™˜๐™š ๐™– ๐™จ๐™ž๐™ฃ๐™œ๐™ก๐™š ๐™ฉ๐™ฎ๐™ฅ๐™š ๐™ค๐™› ๐™˜๐™š๐™ก๐™ก, ๐™ค๐™ง ๐™ข๐™ช๐™ก๐™ฉ๐™ž๐™ฅ๐™ก๐™š ๐™จ๐™ช๐™—๐™ฅ๐™ค๐™ฅ๐™ช๐™ก๐™–๐™ฉ๐™ž๐™ค๐™ฃ๐™จ, ๐™จ๐™ค๐™ข๐™š ๐™ฉ๐™๐™–๐™ฉ ๐™–๐™ง๐™š ๐™ฅ๐™ช๐™ง๐™š๐™ก๐™ฎ ๐™ข๐™š๐™จ๐™š๐™ฃ๐™˜๐™๐™ฎ๐™ข๐™–๐™ก, ๐™–๐™ฃ๐™™ ๐™ค๐™ฉ๐™๐™š๐™ง๐™จ ๐™ฉ๐™๐™–๐™ฉ ๐™–๐™ง๐™š ๐™—๐™ค๐™ฉ๐™ ๐™ข๐™š๐™จ๐™š๐™ฃ๐™˜๐™๐™ฎ๐™ข๐™–๐™ก ๐™–๐™ฃ๐™™ ๐™จ๐™ฉ๐™š๐™ข-๐™ก๐™ž๐™ ๐™š?’ Understanding this will help diagnose and selectively target these aggressive stem-like cancer cells.

This question was explored brilliantly by Petra den Hollander, and members of my team uncovered:

  • ๐™’๐™๐™š๐™ฃ ๐™˜๐™–๐™ฃ๐™˜๐™š๐™ง ๐™˜๐™š๐™ก๐™ก๐™จ ๐™ช๐™ฃ๐™™๐™š๐™ง๐™œ๐™ค ๐™€๐™ˆ๐™, ๐™–๐™ก๐™ก ๐™œ๐™–๐™ž๐™ฃ ๐™ข๐™š๐™จ๐™š๐™ฃ๐™˜๐™๐™ฎ๐™ข๐™–๐™ก ๐™˜๐™๐™–๐™ง๐™–๐™˜๐™ฉ๐™š๐™ง๐™ž๐™จ๐™ฉ๐™ž๐™˜๐™จ, ๐™—๐™ช๐™ฉ ๐™ค๐™ฃ๐™ก๐™ฎ ๐™– ๐™จ๐™ช๐™—๐™จ๐™š๐™ฉ ๐™–๐™˜๐™ฆ๐™ช๐™ž๐™ง๐™š๐™จ ๐™จ๐™ฉ๐™š๐™ข๐™ฃ๐™š๐™จ๐™จ.
  • ๐™€๐™ซ๐™š๐™ง๐™ฎ ๐™จ๐™ฉ๐™š๐™ข-๐™ก๐™ž๐™ ๐™š ๐™˜๐™š๐™ก๐™ก ๐™™๐™ž๐™จ๐™ฅ๐™ก๐™–๐™ฎ๐™จ ๐™ข๐™š๐™จ๐™š๐™ฃ๐™˜๐™๐™ฎ๐™ข๐™–๐™ก ๐™ฉ๐™ง๐™–๐™ž๐™ฉ๐™จ, ๐™—๐™ช๐™ฉ ๐™ฃ๐™ค๐™ฉ ๐™š๐™ซ๐™š๐™ง๐™ฎ ๐™ข๐™š๐™จ๐™š๐™ฃ๐™˜๐™๐™ฎ๐™ข๐™–๐™ก ๐™˜๐™š๐™ก๐™ก ๐™œ๐™–๐™ž๐™ฃ๐™จ ๐™จ๐™ฉ๐™š๐™ข๐™ฃ๐™š๐™จ๐™จ.
  • ๐™Ž๐™ฉ๐™š๐™ข ๐™˜๐™š๐™ก๐™ก ๐™ฅ๐™ง๐™ค๐™ฅ๐™š๐™ง๐™ฉ๐™ž๐™š๐™จ ๐™ง๐™š๐™ฆ๐™ช๐™ž๐™ง๐™š ๐™˜๐™š๐™ก๐™ก ๐™™๐™ž๐™ซ๐™ž๐™จ๐™ž๐™ค๐™ฃ, ๐™ฌ๐™๐™š๐™ง๐™š๐™–๐™จ ๐™ข๐™š๐™จ๐™š๐™ฃ๐™˜๐™๐™ฎ๐™ข๐™–๐™ก ๐™ฉ๐™ง๐™–๐™ž๐™ฉ๐™จ ๐™˜๐™–๐™ฃ ๐™–๐™ง๐™ž๐™จ๐™š ๐™จ๐™ž๐™ข๐™ฅ๐™ก๐™ฎ ๐™›๐™ง๐™ค๐™ข ๐™š๐™ญ๐™ฅ๐™ค๐™จ๐™ช๐™ง๐™š ๐™ฉ๐™ค ๐™€๐™ˆ๐™-๐™ž๐™ฃ๐™™๐™ช๐™˜๐™ž๐™ฃ๐™œ ๐™จ๐™ž๐™œ๐™ฃ๐™–๐™ก๐™จ, ๐™ฌ๐™ž๐™ฉ๐™๐™ค๐™ช๐™ฉ ๐™ฅ๐™ง๐™ค๐™ก๐™ž๐™›๐™š๐™ง๐™–๐™ฉ๐™ž๐™ค๐™ฃ.
  • ๐˜ผ๐™ก๐™ฉ๐™š๐™ง๐™ฃ๐™–๐™ฉ๐™ž๐™ซ๐™š ๐™จ๐™ฅ๐™ก๐™ž๐™˜๐™ž๐™ฃ๐™œ ๐™ง๐™š๐™œ๐™ช๐™ก๐™–๐™ฉ๐™š๐™จ ๐™ฉ๐™๐™š ๐™–๐™˜๐™ฆ๐™ช๐™ž๐™จ๐™ž๐™ฉ๐™ž๐™ค๐™ฃ ๐™ค๐™› ๐™จ๐™ฉ๐™š๐™ข๐™ฃ๐™š๐™จ๐™จ ๐™ž๐™ฃ ๐™– ๐™˜๐™š๐™ก๐™ก ๐™™๐™ž๐™ซ๐™ž๐™จ๐™ž๐™ค๐™ฃโ€“๐™™๐™š๐™ฅ๐™š๐™ฃ๐™™๐™š๐™ฃ๐™ฉ ๐™ข๐™–๐™ฃ๐™ฃ๐™š๐™ง.

These insights remind us that biology is rarely binary. Even within this EMT pathway, multiple cell states exist, each with distinct potential for tumor evolution and therapy resistance. Understanding this diversity is the key to designing more precise and effective cancer treatments.”

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