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Sendoel Lab Investigates the Impact of Aging on Stem Cells’ Regenerative Capacity
Nov 10, 2024, 17:06

Sendoel Lab Investigates the Impact of Aging on Stem Cells’ Regenerative Capacity

Sendoel Lab posted the following on X:

“How does aging alter the translational landscape of stem cells and how do these changes impact their regenerative capacity? Here we developed an in vivo single-cell ribosome profiling strategy to monitor translational landscapes of the mouse skin.

Sendoel Lab Investigates the Impact of Aging on Stem Cells’ Regenerative Capacity

Building on the elegant single-cell protocol by the van Oudenaarden group, we introduced two key modifications: ribosomal elongation-inhibited epidermis isolation and switching to RNase I ribonuclease for generating ribosomal footprints, as commonly used in bulk Ribo-seq.

Sendoel Lab Investigates the Impact of Aging on Stem Cells’ Regenerative Capacity

Using our RNase I-based in vivo ribosome profiling strategy, we observed excellent triplet periodicity – a hallmark of high-quality data – across diverse epidermal single cells, including epidermal stem cells, hair follicle cells and T cells.

Sendoel Lab Investigates the Impact of Aging on Stem Cells’ Regenerative Capacity

Combining scRNA-seq and scRibo-seq, we map the in vivo translational landscapes of major epidermal cell types and detail aging-specific shifts in translational efficiencies. We uncover AP-1 reprogramming specifically in aged epidermal stem cells, impacting keratinocyte behavior.

Sendoel Lab Investigates the Impact of Aging on Stem Cells’ Regenerative Capacity Sendoel Lab Investigates the Impact of Aging on Stem Cells’ Regenerative Capacity

AP-1 levels in aging drive inflammatory responses and inflammaging. AP-1 has also been reported to play a critical role in chromatin opening, thereby altering cellular function and prompting further studies into the posttranscriptional regulation of AP-1 in aged stem cells.

Our study – led by the talented Clara Duré and in collaboration with the Ellis and Levesque labs – suggests a critical role of AP-1 translation in aged stem cells and offers a scalable strategy for tissue-wide interrogation of translational landscapes at single-cell resolution.”

In vivo single-cell ribosome profiling reveals cell-type-specific translational programs during aging

Authors: Clara Duré, Umesh Ghoshdastider, Ramona Weber, Fabiola Valdivia-Francia, Peter F. Renz, Ameya Khandekar, Federica Sella, Katie Hyams, David Taborsky, Merve Yigit, Mark Ormiston, Homare Yamahachi, Mitchell Levesque, Stephanie Ellis, Ataman Sendoel

Sendoel Lab Investigates the Impact of Aging on Stem Cells’ Regenerative Capacity